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KMID : 0848120120370030109
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International Journal of Oral Biology
2012 Volume.37 No. 3 p.109 ~ p.114
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Identification of differentially expressed genes using an annealing control primer system in periodontitis
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Chung Jin
Na Hee-Sam
Kim Ji-S
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Abstract
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In the gingival tissues of patients with periodontitis,inflammatory responses are mediated by a wide variety ofgenes. In this study, we screened for differentially expressedgenes (DEGs) in periodontitis compared with normaltissue using an annealing control primer (ACP) system. ByACP RT-PCR analysis, we obtained about 160 amplicons,8 of which were found to be differentially expressed. DEGsin patients with periodontitis were thus successfully andreliably identified by the ACP-based RT PCR technique.The DEGs identified in the screen may also enhance ourunderstanding of the pathogenesis of periodontitis.
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KEYWORD
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periodontitis ACP-PCR, differentially expressed genes
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